目的 研究藿香正气水的快速质量评价方法。方法 采用超高效液相色谱结合波长切换技术同时测定藿香正气水中橙皮苷、甘草酸铵、欧前胡素、和厚朴酚、异欧前胡素、厚朴酚、苍术素的含量。样品不经处理直接进样。采用ACQUITY UPLC BEH C₁₈色谱柱(2.1 mm×100 mm,1.8 μm)分离,柱温40 ℃。乙腈-0.1%磷酸梯度洗脱,流速0.3 mL·min^-1。284 nm下检测橙皮苷,250 nm下检测甘草酸铵,300 nm下检测欧前胡素、和厚朴酚、异欧前胡素、厚朴酚,340 nm下检测苍术素。结果 7个成分在各自范围内线性关系良好。橙皮苷、甘草酸铵、欧前胡素、和厚朴酚、异欧前胡素、厚朴酚和苍术素的平均回收率分别为99.3%、99.5%、101.5%、99.3%、100.6%、99.0%和99.6%。13个厂家的28批样品甘草酸铵、欧前胡素、异欧前胡素、苍术素的含量差异较大。结论 所建方法准确、简便、快速,可为藿香正气水的全面质量控制提供参考。

OBJECTIVE To investigate a rapid approach for quality evaluation of Huoxiang Zhengqi Tincture. METHODS Hesperidin, glycyrrhizic acid, imperatorin, honokiol, isoimperatorin, magnolol and atractylodin in Huoxiang Zhengqi Tincture were determined by ultra performance liquid chromatography (UPLC) method combined with wavelength switching detection technology. The sample was injected directly without preprocessing. The separation was performed on an ACQUITY UPLC BEH C₁₈ (2.1 mm×100 mm, 1.8 μm) and the column temperature was maitained at 40 ℃. The mobile phase was composed of acetonitrile and 0.1% phosphoric acid with gradient elution at a flaw rate of 0.3 mL·min^-1. Hesperidin was detected at 284 nm; glycyrrhizic acid was detected at 250 nm; imperatorin, honokiol, isoimperatorin and magnolol were detected at 300 nm; atractylodin was detected at 340 nm. RESULTS The calibration curves of the seven components showed good linearity within their test ranges. The average recoveries for hesperidin, glycyrrhizic acid, imperatorin, honokiol, isoimperatorin, magnolol and atractylodin were 99.3%, 99.5%, 101.5%, 99.3%, 100.6%, 99.0% and 99.6%, respectively. And there were great variations among the contents of glycyrrhizic acid, imperatorin, isoimperatorin and atractylodin in 28 batches of samples from 13 manufactures. CONCLUSION The proposed method is accurate, simple and rapid, thus providing basis for comprehensive quality control of Huoxiang Zhengqi Tincture.